Review



anti mouse cd4 violet fluor tm 450  (Cytek Biosciences)


Bioz Verified Symbol Cytek Biosciences is a verified supplier
Bioz Manufacturer Symbol Cytek Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 92
      Buy from Supplier

    Structured Review

    Cytek Biosciences anti mouse cd4 violet fluor tm 450
    a UMAP plot showing 3 clusters of 2967 macrophages (indicated by colors). b Heatmap of signature genes for macrophages clusters. Each cell cluster is represented by four specifically expressed genes. c KEGG pathway enrichment analysis using the characteristic genes of macrophage subpopulation. d Bar plots of number of the subclusters of macrophages in control and cKO male mice. e Volcano plot displaying the −Log10 P vs Log2 fold-change of genes differentially expressed between control and cKO in macrophages. f KEGG pathway enrichment analysis using the intergroup differential gene of macrophages. g Western blot of CLTC, LIPA, LAPTM5 and LAMP2 in macrophages from control and cKO groups using GAPDH as loading control. h Western blot of CCR1, CCR5, CX3CR1 and CXCR4 in macrophages from control and cKO groups using GAPDH as loading control. i Representative flow cytometry plots (left) and statistical analysis of MHCII + macrophages (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. j Western blot of CXCL9 in MHCII + TAMs and MHCII - TAMs using GAPDH as loading control. k Representative flow cytometry plots (left) and statistical analysis of CD3 + <t>CD4</t> + T cells and CD3 + CD8 + cells (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. l Representative immunofluorescence (IF) staining images of CD8 (red) and GZMA (green, right). Statistical analysis of the ratio of CD8 + GZMA + cells to CD8 + cells (CD8T killing capacity) in control and cKO male mice (left). Scale bar, 50 μm. Data are expressed as mean ± SD ( n = 6). P values were calculated by two-tailed unpaired Student’s t test.
    Anti Mouse Cd4 Violet Fluor Tm 450, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+violet+fluor/pmc10985117-385-50-57?v=Cytek+Biosciences
    Average 92 stars, based on 12 article reviews
    anti mouse cd4 violet fluor tm 450 - by Bioz Stars, 2026-08
    92/100 stars

    Images

    1) Product Images from "CD276-dependent efferocytosis by tumor-associated macrophages promotes immune evasion in bladder cancer"

    Article Title: CD276-dependent efferocytosis by tumor-associated macrophages promotes immune evasion in bladder cancer

    Journal: Nature Communications

    doi: 10.1038/s41467-024-46735-5

    a UMAP plot showing 3 clusters of 2967 macrophages (indicated by colors). b Heatmap of signature genes for macrophages clusters. Each cell cluster is represented by four specifically expressed genes. c KEGG pathway enrichment analysis using the characteristic genes of macrophage subpopulation. d Bar plots of number of the subclusters of macrophages in control and cKO male mice. e Volcano plot displaying the −Log10 P vs Log2 fold-change of genes differentially expressed between control and cKO in macrophages. f KEGG pathway enrichment analysis using the intergroup differential gene of macrophages. g Western blot of CLTC, LIPA, LAPTM5 and LAMP2 in macrophages from control and cKO groups using GAPDH as loading control. h Western blot of CCR1, CCR5, CX3CR1 and CXCR4 in macrophages from control and cKO groups using GAPDH as loading control. i Representative flow cytometry plots (left) and statistical analysis of MHCII + macrophages (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. j Western blot of CXCL9 in MHCII + TAMs and MHCII - TAMs using GAPDH as loading control. k Representative flow cytometry plots (left) and statistical analysis of CD3 + CD4 + T cells and CD3 + CD8 + cells (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. l Representative immunofluorescence (IF) staining images of CD8 (red) and GZMA (green, right). Statistical analysis of the ratio of CD8 + GZMA + cells to CD8 + cells (CD8T killing capacity) in control and cKO male mice (left). Scale bar, 50 μm. Data are expressed as mean ± SD ( n = 6). P values were calculated by two-tailed unpaired Student’s t test.
    Figure Legend Snippet: a UMAP plot showing 3 clusters of 2967 macrophages (indicated by colors). b Heatmap of signature genes for macrophages clusters. Each cell cluster is represented by four specifically expressed genes. c KEGG pathway enrichment analysis using the characteristic genes of macrophage subpopulation. d Bar plots of number of the subclusters of macrophages in control and cKO male mice. e Volcano plot displaying the −Log10 P vs Log2 fold-change of genes differentially expressed between control and cKO in macrophages. f KEGG pathway enrichment analysis using the intergroup differential gene of macrophages. g Western blot of CLTC, LIPA, LAPTM5 and LAMP2 in macrophages from control and cKO groups using GAPDH as loading control. h Western blot of CCR1, CCR5, CX3CR1 and CXCR4 in macrophages from control and cKO groups using GAPDH as loading control. i Representative flow cytometry plots (left) and statistical analysis of MHCII + macrophages (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. j Western blot of CXCL9 in MHCII + TAMs and MHCII - TAMs using GAPDH as loading control. k Representative flow cytometry plots (left) and statistical analysis of CD3 + CD4 + T cells and CD3 + CD8 + cells (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. l Representative immunofluorescence (IF) staining images of CD8 (red) and GZMA (green, right). Statistical analysis of the ratio of CD8 + GZMA + cells to CD8 + cells (CD8T killing capacity) in control and cKO male mice (left). Scale bar, 50 μm. Data are expressed as mean ± SD ( n = 6). P values were calculated by two-tailed unpaired Student’s t test.

    Techniques Used: Control, Western Blot, Flow Cytometry, Two Tailed Test, Immunofluorescence, Staining



    Similar Products

    violet 450 anti mouse cd4 antibody  (Elabscience Biotechnology)
    93
    Elabscience Biotechnology violet 450 anti mouse cd4 antibody
    Violet 450 Anti Mouse Cd4 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+violet+fluor/pm40838789-268-29-42?v=Elabscience+Biotechnology
    Average 93 stars, based on 1 article reviews
    violet 450 anti mouse cd4 antibody - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier
    anti mouse cd4 violet fluor tm 450  (Cytek Biosciences)
    92
    Cytek Biosciences anti mouse cd4 violet fluor tm 450
    a UMAP plot showing 3 clusters of 2967 macrophages (indicated by colors). b Heatmap of signature genes for macrophages clusters. Each cell cluster is represented by four specifically expressed genes. c KEGG pathway enrichment analysis using the characteristic genes of macrophage subpopulation. d Bar plots of number of the subclusters of macrophages in control and cKO male mice. e Volcano plot displaying the −Log10 P vs Log2 fold-change of genes differentially expressed between control and cKO in macrophages. f KEGG pathway enrichment analysis using the intergroup differential gene of macrophages. g Western blot of CLTC, LIPA, LAPTM5 and LAMP2 in macrophages from control and cKO groups using GAPDH as loading control. h Western blot of CCR1, CCR5, CX3CR1 and CXCR4 in macrophages from control and cKO groups using GAPDH as loading control. i Representative flow cytometry plots (left) and statistical analysis of MHCII + macrophages (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. j Western blot of CXCL9 in MHCII + TAMs and MHCII - TAMs using GAPDH as loading control. k Representative flow cytometry plots (left) and statistical analysis of CD3 + <t>CD4</t> + T cells and CD3 + CD8 + cells (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. l Representative immunofluorescence (IF) staining images of CD8 (red) and GZMA (green, right). Statistical analysis of the ratio of CD8 + GZMA + cells to CD8 + cells (CD8T killing capacity) in control and cKO male mice (left). Scale bar, 50 μm. Data are expressed as mean ± SD ( n = 6). P values were calculated by two-tailed unpaired Student’s t test.
    Anti Mouse Cd4 Violet Fluor Tm 450, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+violet+fluor/pmc10985117-385-50-57?v=Cytek+Biosciences
    Average 92 stars, based on 1 article reviews
    anti mouse cd4 violet fluor tm 450 - by Bioz Stars, 2026-08
    92/100 stars
    		  Buy from Supplier
    cd4 violet fluor  (Cytek Biosciences)
    93
    Cytek Biosciences cd4 violet fluor
    a UMAP plot showing 3 clusters of 2967 macrophages (indicated by colors). b Heatmap of signature genes for macrophages clusters. Each cell cluster is represented by four specifically expressed genes. c KEGG pathway enrichment analysis using the characteristic genes of macrophage subpopulation. d Bar plots of number of the subclusters of macrophages in control and cKO male mice. e Volcano plot displaying the −Log10 P vs Log2 fold-change of genes differentially expressed between control and cKO in macrophages. f KEGG pathway enrichment analysis using the intergroup differential gene of macrophages. g Western blot of CLTC, LIPA, LAPTM5 and LAMP2 in macrophages from control and cKO groups using GAPDH as loading control. h Western blot of CCR1, CCR5, CX3CR1 and CXCR4 in macrophages from control and cKO groups using GAPDH as loading control. i Representative flow cytometry plots (left) and statistical analysis of MHCII + macrophages (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. j Western blot of CXCL9 in MHCII + TAMs and MHCII - TAMs using GAPDH as loading control. k Representative flow cytometry plots (left) and statistical analysis of CD3 + <t>CD4</t> + T cells and CD3 + CD8 + cells (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. l Representative immunofluorescence (IF) staining images of CD8 (red) and GZMA (green, right). Statistical analysis of the ratio of CD8 + GZMA + cells to CD8 + cells (CD8T killing capacity) in control and cKO male mice (left). Scale bar, 50 μm. Data are expressed as mean ± SD ( n = 6). P values were calculated by two-tailed unpaired Student’s t test.
    Cd4 Violet Fluor, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+violet+fluor/pm36157587-172-25-28?v=Cytek+Biosciences
    Average 93 stars, based on 1 article reviews
    cd4 violet fluor - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier
    cd4 violet fluor 450 rm4 5 tonbo biosciences annexin v pe tonbo biosciences 7aad tonbo biosciences flica panel antibody marker fluorophore  (Cytek Biosciences)
    96
    Cytek Biosciences cd4 violet fluor 450 rm4 5 tonbo biosciences annexin v pe tonbo biosciences 7aad tonbo biosciences flica panel antibody marker fluorophore
    a UMAP plot showing 3 clusters of 2967 macrophages (indicated by colors). b Heatmap of signature genes for macrophages clusters. Each cell cluster is represented by four specifically expressed genes. c KEGG pathway enrichment analysis using the characteristic genes of macrophage subpopulation. d Bar plots of number of the subclusters of macrophages in control and cKO male mice. e Volcano plot displaying the −Log10 P vs Log2 fold-change of genes differentially expressed between control and cKO in macrophages. f KEGG pathway enrichment analysis using the intergroup differential gene of macrophages. g Western blot of CLTC, LIPA, LAPTM5 and LAMP2 in macrophages from control and cKO groups using GAPDH as loading control. h Western blot of CCR1, CCR5, CX3CR1 and CXCR4 in macrophages from control and cKO groups using GAPDH as loading control. i Representative flow cytometry plots (left) and statistical analysis of MHCII + macrophages (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. j Western blot of CXCL9 in MHCII + TAMs and MHCII - TAMs using GAPDH as loading control. k Representative flow cytometry plots (left) and statistical analysis of CD3 + <t>CD4</t> + T cells and CD3 + CD8 + cells (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. l Representative immunofluorescence (IF) staining images of CD8 (red) and GZMA (green, right). Statistical analysis of the ratio of CD8 + GZMA + cells to CD8 + cells (CD8T killing capacity) in control and cKO male mice (left). Scale bar, 50 μm. Data are expressed as mean ± SD ( n = 6). P values were calculated by two-tailed unpaired Student’s t test.
    Cd4 Violet Fluor 450 Rm4 5 Tonbo Biosciences Annexin V Pe Tonbo Biosciences 7aad Tonbo Biosciences Flica Panel Antibody Marker Fluorophore, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+violet+fluor/pm35668170__41565_2022_1137_MOESM1_ESM-123-39-44?v=Cytek+Biosciences
    Average 96 stars, based on 1 article reviews
    cd4 violet fluor 450 rm4 5 tonbo biosciences annexin v pe tonbo biosciences 7aad tonbo biosciences flica panel antibody marker fluorophore - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier
    bsa violet fluor 450 conjugated ab anti cd4  (Cytek Biosciences)
    93
    Cytek Biosciences bsa violet fluor 450 conjugated ab anti cd4
    a UMAP plot showing 3 clusters of 2967 macrophages (indicated by colors). b Heatmap of signature genes for macrophages clusters. Each cell cluster is represented by four specifically expressed genes. c KEGG pathway enrichment analysis using the characteristic genes of macrophage subpopulation. d Bar plots of number of the subclusters of macrophages in control and cKO male mice. e Volcano plot displaying the −Log10 P vs Log2 fold-change of genes differentially expressed between control and cKO in macrophages. f KEGG pathway enrichment analysis using the intergroup differential gene of macrophages. g Western blot of CLTC, LIPA, LAPTM5 and LAMP2 in macrophages from control and cKO groups using GAPDH as loading control. h Western blot of CCR1, CCR5, CX3CR1 and CXCR4 in macrophages from control and cKO groups using GAPDH as loading control. i Representative flow cytometry plots (left) and statistical analysis of MHCII + macrophages (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. j Western blot of CXCL9 in MHCII + TAMs and MHCII - TAMs using GAPDH as loading control. k Representative flow cytometry plots (left) and statistical analysis of CD3 + <t>CD4</t> + T cells and CD3 + CD8 + cells (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. l Representative immunofluorescence (IF) staining images of CD8 (red) and GZMA (green, right). Statistical analysis of the ratio of CD8 + GZMA + cells to CD8 + cells (CD8T killing capacity) in control and cKO male mice (left). Scale bar, 50 μm. Data are expressed as mean ± SD ( n = 6). P values were calculated by two-tailed unpaired Student’s t test.
    Bsa Violet Fluor 450 Conjugated Ab Anti Cd4, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+violet+fluor/pmc06727150-54-18-26?v=Cytek+Biosciences
    Average 93 stars, based on 1 article reviews
    bsa violet fluor 450 conjugated ab anti cd4 - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    a UMAP plot showing 3 clusters of 2967 macrophages (indicated by colors). b Heatmap of signature genes for macrophages clusters. Each cell cluster is represented by four specifically expressed genes. c KEGG pathway enrichment analysis using the characteristic genes of macrophage subpopulation. d Bar plots of number of the subclusters of macrophages in control and cKO male mice. e Volcano plot displaying the −Log10 P vs Log2 fold-change of genes differentially expressed between control and cKO in macrophages. f KEGG pathway enrichment analysis using the intergroup differential gene of macrophages. g Western blot of CLTC, LIPA, LAPTM5 and LAMP2 in macrophages from control and cKO groups using GAPDH as loading control. h Western blot of CCR1, CCR5, CX3CR1 and CXCR4 in macrophages from control and cKO groups using GAPDH as loading control. i Representative flow cytometry plots (left) and statistical analysis of MHCII + macrophages (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. j Western blot of CXCL9 in MHCII + TAMs and MHCII - TAMs using GAPDH as loading control. k Representative flow cytometry plots (left) and statistical analysis of CD3 + CD4 + T cells and CD3 + CD8 + cells (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. l Representative immunofluorescence (IF) staining images of CD8 (red) and GZMA (green, right). Statistical analysis of the ratio of CD8 + GZMA + cells to CD8 + cells (CD8T killing capacity) in control and cKO male mice (left). Scale bar, 50 μm. Data are expressed as mean ± SD ( n = 6). P values were calculated by two-tailed unpaired Student’s t test.

    Journal: Nature Communications

    Article Title: CD276-dependent efferocytosis by tumor-associated macrophages promotes immune evasion in bladder cancer

    doi: 10.1038/s41467-024-46735-5

    Figure Lengend Snippet: a UMAP plot showing 3 clusters of 2967 macrophages (indicated by colors). b Heatmap of signature genes for macrophages clusters. Each cell cluster is represented by four specifically expressed genes. c KEGG pathway enrichment analysis using the characteristic genes of macrophage subpopulation. d Bar plots of number of the subclusters of macrophages in control and cKO male mice. e Volcano plot displaying the −Log10 P vs Log2 fold-change of genes differentially expressed between control and cKO in macrophages. f KEGG pathway enrichment analysis using the intergroup differential gene of macrophages. g Western blot of CLTC, LIPA, LAPTM5 and LAMP2 in macrophages from control and cKO groups using GAPDH as loading control. h Western blot of CCR1, CCR5, CX3CR1 and CXCR4 in macrophages from control and cKO groups using GAPDH as loading control. i Representative flow cytometry plots (left) and statistical analysis of MHCII + macrophages (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. j Western blot of CXCL9 in MHCII + TAMs and MHCII - TAMs using GAPDH as loading control. k Representative flow cytometry plots (left) and statistical analysis of CD3 + CD4 + T cells and CD3 + CD8 + cells (right) in control and cKO groups. Data are presented as mean ± SD ( n = 6). P value was calculated by two-tailed unpaired Student’s t test. l Representative immunofluorescence (IF) staining images of CD8 (red) and GZMA (green, right). Statistical analysis of the ratio of CD8 + GZMA + cells to CD8 + cells (CD8T killing capacity) in control and cKO male mice (left). Scale bar, 50 μm. Data are expressed as mean ± SD ( n = 6). P values were calculated by two-tailed unpaired Student’s t test.

    Article Snippet: Anti-Mouse CD11C PE-CY7 (25-0114-81, ThermoFisher, 1:200); Anti-Mouse F4/80 PCP-CY5.5 (45-4801-80, Thermo Fisher, 1:200); Anti-Mouse Ly-6G (Gr-1) Alexa Fluor® 700 (56-5931-82, ThermoFisher, 1:200); Annexin V-APC/7-AAD Apoptosis kit (AP105, LIANKE, 1:200); Anti-Mouse MHC Class II (I-A/I-E) eFluor® 450 (48-5321-82, ThermoFisher, 1:200); Anti-Mouse CD45 PE-CY7(60-0451, Tonbo, 1:200); Anti-Mouse CD3 APC (20-0032, Tonbo, 1:200); Anti-Mouse CD4 violet Fluor TM 450 (75-0041, Tonbo, 1:200); Anti-Mouse CD8a FITC(35-0081, Tonbo, 1:200) and Ghost Dye TM Red 780 (13-0865, Tonbo, 1:100) were the antibodies and dyes used for flow cytometry (CytoFLEX, Beckman Coulter).

    Techniques: Control, Western Blot, Flow Cytometry, Two Tailed Test, Immunofluorescence, Staining